Update README.md

README.md

@@ -106,6 +106,17 @@ All details can be found in the script [scGate_Fig2C_dataset_to_Fig2H_dataset.R]
 
 #### WPRE quantification and mapping 
 
-
+To evaluate the transduction efficiency of Fig2H patients samples, we assessed for expression
+of WPRE (LV specific gene) transcripts as surrogate of the IDUA LV-derived transgene, which
+itself could not be discriminated by IDUA transcripts originating from the native gene. Alignment
+and quantification of WPRE transcripts was ensured by adding the WPRE sequence to the
+reference human genome in cellranger (10X Genomics). Transduction efficiency (TE) was hence 
+calculated as the fraction of cells bearing the WPRE transcripts within each sample and/or cell
+population.
+
+Code for performing quantification, mapping and quantification can be found in:  
+
+- 
+- [Fig2H.R](http://www.bioinfotiget.it/gitlab/custom/zonari_mpbhscexp_2025/zonari_mpbhscexp_2025_scrnaseq/-/blob/main/Fig2H.R)