@@ -34,19 +34,18 @@ HSC can engraft and allow long-term disease correction.
...
@@ -34,19 +34,18 @@ HSC can engraft and allow long-term disease correction.
In the main folder the main function for processing data produced by Barseq pipeline [Ferrari, Beretta et al, 2021](https://pubmed.ncbi.nlm.nih.gov/34031609/). Below a glimpse of the file produced:
In the main folder the main function for processing data produced by Barseq pipeline [Ferrari, Beretta et al, 2021](https://pubmed.ncbi.nlm.nih.gov/34031609/). Below a glimpse of the file produced:
`
| Barcode | Count | SaturationPerc |
Barcode Count SaturationPerc
| ------ | ------ | -------------- |
"TCTTTACTATAAAAAA" 19689 6.80
| TCTTTACTATAAAAAA | 19689 | 6.80 |
"GGATGTCCCTTATAAT" 17875 12.97
| GGATGTCCCTTATAAT | 17875 | 12.97 |
"TTAGGTTGTAATCATT" 14521 17.99
| TTAGGTTGTAATCATT | 14521 | 17.99 |
"CGGAATAATTATTGGC" 14363 22.95
| CGGAATAATTATTGGC | 14363 | 22.95 |
"ATATTTACTATAACAC" 13786 27.71
| ATATTTACTATAACAC | 13786 | 27.71 |
"TAGGATGAAACATTTG" 13764 32.46
| TAGGATGAAACATTTG | 13764 | 32.46 |
"TTACTAATTAATCTTA" 13425 37.10
| TTACTAATTAATCTTA | 13425 | 37.10 |
"TAAATTCATACAATCC" 5939 39.15
| TAAATTCATACAATCC | 5939 | 39.15 |
"ACTGATGCTTCACGAA" 3067 40.21
| ACTGATGCTTCACGAA | 3067 | 40.21 |
`
This file (one for each sample) are then mainly processed by using the custom function [Charting_BCs_Light.R](http://www.bioinfotiget.it/gitlab/custom/zonari_mpbhscexp_2025/zonari_mpbhscexp_2025_barcoding/-/blob/main/ChartingBCs_Light.R) script and futherly analyzed with scripts present in each folder (one script for each experiment):
This file (one for each sample) are then mainly processed by using the custom function [Charting_BCs_Light.R](http://www.bioinfotiget.it/gitlab/custom/zonari_mpbhscexp_2025/zonari_mpbhscexp_2025_barcoding/-/blob/main/ChartingBCs_Light.R) script and futherly analyzed with scripts present in each folder (one script for each experiment):
