From f9222341325ef27e71dd10c46ded216854a49410 Mon Sep 17 00:00:00 2001 From: Giorgia Giacomini Date: Wed, 18 Dec 2024 16:37:00 +0000 Subject: [PATCH] Update README.md --- README.md | 10 +--------- 1 file changed, 1 insertion(+), 9 deletions(-) diff --git a/README.md b/README.md index 65f3b29..cac166e 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,5 @@ # Amodio_Infertility_2023 -Amodio G, Giacomini G, Boeri L, et al. **T cell exhaustion and senescence signatures characterize and differentiate infertile men** +Amodio G, Giacomini G, Boeri L, et al. **Specific types of male infertility are correlated with T cell exhaustion or senescence signaturesn** ### Single-Cell RNA Sequencing Analysis ### **scRNAseq** (from 10X Genomics) analysis of CD3+ T cells purified from the peripheral blood of men diagnosed with oligo-astheno-teratozoospermia (OAT, n=4), idiopathic non-obstructive azoospermia (iNOA, n=6), and a control group (FER, n=5). @@ -28,14 +28,6 @@ scRNAseq analysis was performed using a standard [Seurat](https://satijalab.org/ ### Directories and Files ### - sampleSheet.csv: names of samples and corresponding conditions -- **Script**: R scripts used for the analyses - - `1_PreProcessing_Data.R`: Preprocessing, cell filtering and Full object creation - - `2_Infertility_scRNAseq_analysis.R`: - - `3_SubsetAnalysis_Annotations.R`: subset analyses of T cells and cluster manual annotation - - `4_ScoreAnalysis_TcellSubset.R`: - - `5_Visualization_Export_Data.R`: -- **Data**: results of scRNAseq analysis: - -- GitLab