# RNAseq_ARA_TAZ
RNA-seq Differential Expression, GSEA analysis and plots

This repository contains R code for differential expression analysis using DESeq2 and Gene Set Enrichment Analysis (GSEA).

The "data/" directory contains:

- featureCounts_results_replica1.txt – Gene count matrix for replicate 1.
- featureCounts_results_replica2.txt – Gene count matrix for replicate 2.
- Decoder_replica1.txt – Sample annotation (metadata) for replicate 1.
- Decoder_replica2.txt – Sample annotation (metadata) for replicate 2.
- Villani_et_al_GeneSets_DCs.xlsx – Villani et al. dendritic cell gene signatures used for GSEA.
- SenescenceSignatures.txt – Senescence-related gene signatures used for GSEA.

# Citation:

If you use this repository or data, please cite:

Gilioli and Fusco et al., Therapy-Induced Senescence Promotes Immunogenicity in Acute Myeloid Leukemia Blasts through loss of PRC2-Mediated Gene Regulation

Thank you.