diff --git a/README.md b/README.md index 92a24750cdb39e7557aa47233a0214af6ff7501f..0095f4b8e10bdb59f6eb4fbdc9e631455eb13f73 100644 --- a/README.md +++ b/README.md @@ -58,12 +58,12 @@ scRNAseq analysis was performed with [Seurat](https://satijalab.org/seurat/). Be 2. Cell cycle scoring 3. Normalization (default seurat settings) 4. Scaling (with following variables to regress out: percent.mt + nCount_RNA and CC.Difference calculated as show in [vignette](https://satijalab.org/seurat/articles/cell_cycle_vignette.html#alternate-workflow-1)) -4. Dimensionality reduction: PCA -5. Clustering -6. Markers identification +5. Dimensionality reduction: PCA +6. Clustering +7. Markers identification -- [6.1] Clusters related markers -- [6.2] Intracluster differential expression analysis according to comparison of interest +- [7.1] Clusters related markers +- [7.2] Intracluster differential expression analysis according to comparison of interest Pseudotime analysis was performed using [Monocle3](http://cole-trapnell-lab.github.io/monocle3/) and PAGA-tree from [dynverse](https://dynverse.org/)